Sustainable Onshore Lobster Aquaculture
Sustainable Onshore Lobster Aquaculture

Understanding insulin endocrinology in decapod crustacea: Molecular modelling characterization of an insulin-binding protein and insulin-like peptides in the eastern spiny lobster, Sagmariasus verreauxi

Abstract

The insulin signalling system is one of the most conserved endocrine systems of Animalia from mollusc to man. In decapod Crustacea, such as the Eastern spiny lobster, Sagmariasus verreauxi (Sv) and the red-claw crayfish, Cherax quadricarinatus (Cq), insulin endocrinology governs male sexual differentiation through the action of a male-specific, insulin-like androgenic gland peptide (IAG). To understand the bioactivity of IAG it is necessary to consider its bio-regulators such as the insulin-like growth factor binding protein (IGFBP). This work has employed various molecular modelling approaches to represent S. verreauxi IGFBP and IAG, along with additional Sv-ILP ligands, in order to characterise their binding interactions. Firstly, we present Sv-and Cq-ILP2: neuroendocrine factors that share closest homology with Drosophila ILP8 (Dilp8). We then describe the binding interaction of the N-terminal domain of Sv-IGFBP and each ILP through a synergy of computational analyses. In-depth interaction mapping and computational alanine scanning of IGFBP_N’ highlight the conserved involvement of the hotspot residues Q67, G70, D71, S72, G91, G92, T93 and D94. The significance of the negatively charged residues D71 and D94 was then further exemplified by structural electrostatics. The functional importance of the negative surface charge of IGFBP is exemplified in the complementary electropositive charge on the reciprocal binding interface of all three ILP ligands. When examined, this electrostatic complementarity is the inverse of vertebrate homologues, such physicochemical divergences elucidate towards ligand-binding specificity between Phyla. © 2017 by the authors. Licensee MDPI, Basel, Switzerland.

About

Research Hub Investigator(s)
Publication Year
2017
Citation
Chandler, J.C., Gandhi, N.S., Mancera, R.L., Smith, G., Elizur, A. & Ventura, T. 2017, "Understanding insulin endocrinology in decapod crustacea: Molecular modelling characterization of an insulin-binding protein and insulin-like peptides in the eastern spiny lobster, Sagmariasus verreauxi", International Journal of Molecular Sciences, vol. 18, no. 9.
Link
Link to this Publication
Back to our Publication List
Sustainable Onshore Lobster AquacultureSustainable Onshore Lobster Aquaculture
The ARC Research Hub for Sustainable Onshore Lobster Aquaculture is funded by the Australian Government through the Australian Research Council Industrial Transformation Research Program. 

For more information about the Research Hub please contact us at Lobster.Aquaculture@utas.edu.au or phone +61 3 6226 8268.
Australian Research CouncilInstitute of Marine and Antarctic StudiesUniversity of TasmaniaOrnatasUniversity of Sunshine CoastPFG GroupUniversity of New Zealand
Copyright 2021 ARC Research Hub for Sustainable Onshore Lobster Aquaculture.
Top linkedin facebook pinterest youtube rss twitter instagram facebook-blank rss-blank linkedin-blank pinterest youtube twitter instagram