We track visits to the website to tell us how much traffic we are receiving. By closing this window, you agree to our use of cookies. To learn more, click "Manage Cookies"
Accept X
Haemolymph microbiome of the cultured spiny lobster Panulirus ornatus at different temperatures Abstract Lobsters have an open circulatory system with haemolymph that contains microorganisms even in the healthy individuals. Understanding the role of these microorganisms becomes increasingly important particularly for the diagnosis of disease as the closed life-cycle aquaculture of the spiny lobster Panulirus ornatus nears commercial reality. This study aimed to characterise haemolymph responses of healthy cultured P. ornatus juveniles at control (28 °C) and elevated (34 °C) temperatures. This was assessed by measuring immune parameters (total granulocyte counts, total haemocyte counts, clotting times), and culture-independent (pyrosequencing of haemolymph DNA) and culture-dependent (isolation using nonselective growth medium) techniques to analyse bacterial communities from lobster haemolymph sampled on days 0, 4 and 6 post-exposure to the temperature regimes. Elevated temperature (34 °C) affected lobster survival, total granulocyte counts, and diversity, load and functional potential of the haemolymph bacterial community. Pyrosequencing analyses showed that the core haemolymph microbiome consisted of phyla Proteobacteria and Bacteriodetes. Overall, culture-independent methods captured a higher bacterial diversity and load when compared to culture-dependent methods, however members of the Rhodobacteraceae were strongly represented in both analyses. This is the first comprehensive study providing comparisons of haemolymph bacterial communities from healthy and thermally stressed cultured juvenile P. ornatus and has the potential to be used in health monitoring programs. © 2019, The Author(s).
About Research Hub Investigator(s)
Publication Year
2019
Citation
Ooi, M.C., Goulden, E.F., Smith, G.G. & Bridle, A.R. 2019, "Haemolymph microbiome of the cultured spiny lobster Panulirus ornatus at different temperatures", Scientific Reports, vol. 9, no. 1.
Link to this Publication
Back to our Publication List
The ARC Research Hub for Sustainable Onshore Lobster Aquaculture is funded by the Australian Government through the Australian Research Council Industrial Transformation Research Program. +61 3 6226 8268.
Copyright 2024 ARC Research Hub for Sustainable Onshore Lobster Aquaculture.
Top plus close key bars linkedin facebook pinterest youtube instagram facebook-blank linkedin-blank pinterest youtube instagram 
